This difference should be considered whenever an Hct measured on a POC analyzer using electrical conductance is used in chelonian patient assessments.
Istat chem 8 procedure manual#
Hct values measured with electrical conductivity in chelonians significantly underestimated manual PCVs by about 4%. Controlling for other factors, the magnitude of the disagreement was not affected by the sex of the chelonian but was smaller in red-eared sliders (Trachemys scripta elegans). There was a negative correlation between the value of TS and the difference between the methods. Hct significantly underestimated PCV, with a mean difference between the techniques of −3.8% (95% CI: −4.5 to −3.2 LoA: −11.5 to 3.8). Of 192 chelonians sampled during this period, 148 had Hct, PCV, and TS results. Generalized linear mixed models were used to determine the effect of different variables on the difference between Hct and PCV. Bland-Altman plots were built to assess agreement between the measurements. We aimed to assess the agreement between Hct values measured with a POC analyzer and manual PCV measurements in venous samples from 148 chelonians.Īll chelonians that underwent bloodwork for clinical reasons had Hct measured with an analyzer employing electrical conductivity (i-STAT, Abbott), PCVs measured using centrifugation, and total solids (TS) measured with refractometry. A few studies with small sample sizes, evaluating agreement between hematocrit (Hct) and packed cell volume (PCV) measurements in chelonians, showed conflicting results. Point-of-care (POC) analyzers are increasingly used for diagnostic testing in exotic animals. However, clinicians should interpret Po2, creatinine and PT results with caution. The i-STAT Alinity appeared as a convenient device for measurements of numerous parameters. However, correlations of the PT and INR measurements with existing instruments were lower (R2 = 86.0% and 89.7%), and biases in the Po2 (7.9%), creatinine (5.4%) and PT (−6.6%) measurements were higher. We found that the within-lab coefficients of variation (CV) were very low ( 95%) correlated with those of the existing laboratory instruments, and the biases were very low (<2%) or low (2–5%). We assessed the imprecision and compared the results to those obtained on existing instruments in the central laboratory.
We conducted an analytical performances study with the i-STAT Alinity device using cartridges CG4+ (pH, Pco2, Po2, lactate, bicarbonate and base excess) CHEM8+ (Na, K, Cl, ionized Ca, urea, creatinine, glucose, hematocrit and hemoglobin) and PT/INR (prothrombin time and international normalized ratio). Herein, we aimed to assess the analytical performances of the i-STAT Alinity system. However, data regarding their analytical performances in real-world situations remains scarce. Many Point-of-Care devices have been released over the past decade.
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